jdi scoring procedures  (Balzer GmbH)

Journal: FEBS Open Bio

Article Title: Histone demethylase KDM3B protects against ferroptosis by upregulating SLC7A11

doi: 10.1002/2211-5463.12823

Figure Lengend Snippet: The effect of KDM3B overexpression on the Erastin‐induced ferroptosis. (A) The effect of KDM3B in HT‐1080 cells on the ferroptosis induced by Erastin (5 μ m ) and RSL3 (2.5 μ m ). KDM3B stably overexpressed HT‐1080 was constructed as described in the materials and methods. Parental and KDM3B over cells were plated in 96‐well plates at a concentration of 1500 cells per well and incubated with indicated compounds 24 h after plating. Three days later, cells were collected for proliferation detection. (B) The effect of JMJD1C in 293 cells on the ferroptosis induced by Erastin (5 μ m ) and RSL3 (2.5 μ m ). JMJD1C stably overexpressed 293 was constructed as described in the materials and methods. Parental and JMJD1C over cells were plated in 96‐well plates at a concentration of 1500 cells per well and incubated with indicated compounds 24 h after plating. Three days later, cells were collected for proliferation detection. (C) The effect of KDM3B stable overexpression on the H3K9 monomethylation and dimethylation of HT‐1080 cells. Parental and KDM3B over HT‐1080 cells were collected and lysed for Western blot detection. H3 was used as endogenous control. (D) The effect of JMJD1C stable overexpression on the H3K9 monomethylation and dimethylation of 293 cells. Parental and JMJD1C over HT‐1080 cells were collected and lysed for Western blot detection. H3 was used as endogenous control. (E) The effect of KDM3B inhibitor JDI‐16 on Erastin‐induced repression of cell proliferation of THP‐1 cells. THP‐1 cells were plated in 96‐well round‐bottom plates at a concentration of 4000 cells per well and incubated with indicated compounds (Erastin, 5 μ m , JDI‐16, 12.5 μ m ) 24 h after plating. Four days later, cells were collected for proliferation detection. For (A, B, E), three independent experiments were performed, quantitative results are reported as mean ± SD, the statistical analysis was performed using Student's t ‐test, and P value smaller than 0.05 (represented by *) was regarded as statistically significant.

Article Snippet: Erastin was purchased from Sigma (Shanghai, China), RSL3 was purchased from Selleckchem (Shanghai, China), and KDM3B inhibitor JDI‐16 was purchased from Topscience (Shanghai, China).

Techniques: Over Expression, Stable Transfection, Construct, Concentration Assay, Incubation, Western Blot, Control

Journal: FEBS Open Bio

Article Title: Histone demethylase KDM3B protects against ferroptosis by upregulating SLC7A11

doi: 10.1002/2211-5463.12823

Figure Lengend Snippet: The transcriptional regulation of SLC7A11 by KDM3B. (A–F) The mRNA levels of indicated genes affected by KDM3B overexpression. Parental and KDM3B overexpressed HT‐1080 cells were collected for RNA extraction and qPCR. GAPDH was used as an endogenous control. (G) The effect of enforced ATF4, p53, KDM3B, and the combination on the SLC7A11 promoter activities. The SLC7A11 promoter reporter along with ATF4, p53, and KDM3B plasmids was transfected into HT‐1080 cells with pRL‐TK as an endogenous control. (H) The effect of enforced ATF4 and KDM3B siRNA on the SLC7A11 promoter activities. The SLC7A11 promoter reporter along with ATF4 and KDM3B siRNA was transfected into HT‐1080 cells with pRL‐TK as an endogenous control. For (A–H), three independent experiments were performed, quantitative results are reported as mean ± SD, the statistical analysis was performed using Student's t ‐test, and P value smaller than 0.05 (represented by *) was regarded as statistically significant.

Article Snippet: Erastin was purchased from Sigma (Shanghai, China), RSL3 was purchased from Selleckchem (Shanghai, China), and KDM3B inhibitor JDI‐16 was purchased from Topscience (Shanghai, China).

Techniques: Over Expression, RNA Extraction, Control, Transfection

Journal: FEBS Open Bio

Article Title: Histone demethylase KDM3B protects against ferroptosis by upregulating SLC7A11

doi: 10.1002/2211-5463.12823

Figure Lengend Snippet: Schematic representation of the proposed model that characterizes KDM3B's role in Erastin‐induced ferroptosis. Class I FIN (ferroptosis inducer) Erastin and class II FIN RSL3 induce ferroptosis dependent on SLC7A11 and GPX4, respectively. KDM3B transcriptionally upregulates SLC7A11, probably through ATF4 and p53, to confer ferroptosis resistance. KDM3B also represses VDAC3 and CARS. KDM3B inhibitor JDI‐16 could resensitize cells to Erastin‐induced proliferation repression.

Article Snippet: Erastin was purchased from Sigma (Shanghai, China), RSL3 was purchased from Selleckchem (Shanghai, China), and KDM3B inhibitor JDI‐16 was purchased from Topscience (Shanghai, China).

Techniques:

Journal: Scientific Reports

Article Title: Herbal extracts in orofacial pain: a systematic review and direct and indirect meta-analysis

doi: 10.1038/s41598-024-77796-7

Figure Lengend Snippet: Characteristics of included clinical trials investigating herbal extracts for orofacial pain treatment categorized by type of orofacial pain.

Article Snippet: 43 , Komasawa et al., 2018 , SBRCT , Extraction (surgery) , Cinnamomi Cassiae , Syzygium aromaticum , Glycyrrhiza Uralensis , Ligusticum wallichii , Nuphar japonica , Quercus robur , Rheum rhabarbarum , Systemic Jidabokuippo granules , No-Treatment , Patients were given three oral doses (2.5 g each) of JDI (TJ-89, Tsumura Co, Tokyo, Japan) just before falling asleep the night before surgery, and in the morning and around noon on the day of surgery (total 7.5 g)..

Techniques: Clinical Proteomics, Control, Capsules, Isolation, Sterility, Extraction, Medications, Injection, Emulsion, Cream, Stripping Membranes, Adhesive, Ointment, Muscles, Cannabis, Formulation